Arunrat Yangchum a, Thapanee Pruksatrakul a, Malipan Sappan a, Kitlada Srichomthong a, Thitiya Boonpratuang b, Wanrosmani Doloh b, Panida Surawatanawong c, Masahiko Isaka a

a National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Road, Klong Luang, Pathumthani 12120, Thailand

b National Biobank of Thailand, National Science and Technology Development Agency, 111 Thailand Science Park, Phahonyothin Road, Klong Luang, Pathumthani 12120, Thailand

c Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

A highly conjugated 17-nor-neodolastane diterpenoid possessing a nitrile group, hydropusin A (1), and a neodolastane, hydropusin B (2a/2b/2c), were isolated from cultures of the basidiomycete Hydropus cf. Trichoderma TBRC-BCC 43790. Hydropusin B was isolated as a mixture of three forms of interconvertible isomerization, which was supported by a deuteration experiment. Hydropusin A exhibited weak cytotoxicity to NCI–H187 and Vero cell-lines (IC50 60.6 and 64.5 μM, respectively), whereas hydropusin B showed antitubercular activity against Mycobacterium tuberculosis H37Ra (MIC 12.5 μg/mL).

Reference: Yangchum, A.; Pruksatrakul, T.; Sappan, M.; Srichomthong, K.; Boonpratuang, T.; Doloh, W.; Surawatanawong, P.; Isaka, M. Neodolastane diterpenoids from cultures of basidiomycete hydropus cf. trichoderma. Phytochemistry 2026, 250, 114973. https://doi.org/10.1016/j.phytochem.2026.114973.